2024-11-20 00:18 点击次数：81
拳交 小说
一、简介：
赵天永，教养，博士生导师。本科毕业于河北农业大学园艺系。中国农业大学生物学院硕博连读并获作物遗传育种专科博士学位。曾在好意思国U.C.Berkeley和University of Kentucky从事博士后询查并随后任职Scientist I、Scientist II。当前承担本科生《分子生物学》英文教学责任以及博士生的《人命科学询查进展》课程。
二、询查宗旨：玉米抗逆分子生物学
棉子糖是由半乳糖、果糖和葡萄糖结合而成的一种三糖。棉子糖在植物叶片受到窘境威胁时，或在种子练习脱水进程中马上蕴蓄，在植物抗逆、种子活力建成中发扬紧迫作用。由于受到询查材料和询查模式的为止，棉子糖调控植物抗逆和种子活力的作用机制永恒未能笃定。棉子糖的合成需要两步酶促反应来完成。当先肌醇和UDP-半乳糖苷在肌醇半乳糖苷合成酶（Galactinol Synthase，GOLS）作用下酿成肌醇半乳糖苷，然后肌醇半乳糖苷和蔗糖在棉子糖合成酶（Raffinose Synthase，RS）作用下酿成棉子糖。
图1 棉子糖代谢调控过火功能
他东谈主和咱们的询查标明，过量抒发GOLS基因大要擢升肌醇半乳糖苷和棉子糖的含量，擢升植物的抗逆性。但对于GOLS基因反映窘境的转录调控知之甚少。咱们发现了ZmGOLS2基因反映窘境威胁（Zhao, 2003, 2004）,飞动了玉米ZmGOLS2基因运行子上反映热激、脱水等元件(Gu, 2013)，并飞动了在窘境条款下与有关元件结合调控该基因抒发的转录因子，发现过量抒发ZmGOLS2基因大要擢升植物的抗逆性且对植物的滋长莫得负作用(Gu, 2016, 2019; Zhang, 2019)。对于植物RS基因的询查报谈很少，咱们履行室最近克隆到了玉米基因组中惟逐个个棉子糖合成酶基因（ZmRS），发现玉米ZmRS基因的抒发对种子耐老化、植株抗逆起着紧迫作用 (Li, 2017， 2020)。 此外咱们发现转录因子ZmDREB1A径直调控ZmRS基因的抒发，在冷威胁条款下促进棉子糖的合成，擢升植物的抗寒性（Han, 2020）。
α-碱性半乳糖苷酶（AGA）是一类大要识别棉子糖中的α-半乳糖苷键，将棉子糖领悟为蔗糖与半乳糖的糖苷水解酶（图1）。咱们克隆了玉米AGA基因家眷的4个成员，发现 ZmAGA1在种子萌生进程中受高温、低温或脱水威胁和ABA指点抒发(Zhao, 2006； Han, 2015)。AGA基因受窘境威胁指点抒发的生物学兴味兴味尚不了了。
咱们最近发当今玉米不同自交系种子中棉子糖含量存在显赫互异，各自交系限度棉子糖合成和水解的要道酶基因的转录水平，翻译水平，以及所编码卵白的酶催化活性齐存在显赫互异。咱们正在对有关基因的转录调控，翻译调控，酶活调控进行询查。
三、在玉米棉子糖调控种子活力与抗逆领域发表的论文 （第一或通信作家论文）
1. Qinghui Han, Kelu Chen, Dong Yan, Guanglong Hao, Junlong Qi, Chunmei Wang, Lynnette M. Dirk, A. Bruce Downie, Jianhua Gong, Jianhua Wang, Tianyong Zhao*, 2020, ZmDREB2A regulates ZmGH3.2 and ZmRAFS, shifting metabolism towards seed aging tolerance over seedling growth. The Plant Journal. https://onlinelibrary.wiley.com/doi/10. 1111/tpj.14922.
2. Tao Li, Yumin Zhang, Ying Liu, Xudong Li, Guanglong Hao, Qinghui Han, Lynnette M. Dirk, A Bruce Downie, Yong-Ling Ruan, Jianmin Wang, Guoying Wang, Tianyong Zhao*, 2020, Raffinose synthase enhances drought tolerance through raffinose synthesis or galactinol hydrolysis in maize and Arabidopsis plants. Journal of Biological Chemistry. 2020, 295:8064-8077.
3. Qinghui Han, Junlong Qi, Guanglong Hao, Chunxia Zhang, Chunmei Wang, Lynnette M. Dirk, A Bruce Downie, Tianyong Zhao*, 2020, ZmDREB1A regulates RAFFINOSE SYNTHASE controlling raffinose accumulation and plant chilling stress tolerance in maize. Plant and Cell Physiology. 61: 331-341.
4. Lei Gu, Tao Jiang, Chunxia Zhang, Xudong Li, Chunmei Wang, Yumin Zhang, Tao Li, Lynnette Dirk, Bruce Downie, Tianyong Zhao*, 2019, Maize HSFA2 and HSBP2 antagonistically modulate raffinose biosynthesis and heat tolerance in Arabidopsis. The Plant Journal. 100:128-142.
5. Yumin Zhang, Qichao, Sun, Guanglong Hao, Chunmei Wang, Lynnette, Dirk, Bruce Downie, Tianyong Zhao*, 2019, Maize VIVIPAROUS1 Interacts with ABA INSENSITIVE5 to Regulate GALACTINOL SYNTHASE2 Expression Controlling Seed Raffinose Accumulation. J Agric Food Chem. 67(15): 4214-4223.
6. Tao Li, Yumin Zhang, Dong Wang, Ying Liu, Lynnette Dirk, Jack Goodman, Bruce Downie, Jianmin Wang, Guoying Wang, Tianyong Zhao*, 2017, Regulation of seed vigor by manipulation of raffinose family oligosaccharides (RFOs) in maize and Arabidopsis. Molecular Plant. 10(12): 1540-1555.
7. Lei Gu, Yumin Zhang, Mingshuai Zhang, Tao Li, Lynnette M.A. Dirk, Bruce Downie, Tianyong Zhao*, 2016, ZmGOLS2, a target of transcription factor ZmDREB2A, offers similar protection against abiotic stress as ZmDREB2A. Plant Molecular Biology. 90:157-170hesis or galactinol hydrolysis in maize and Arabidopsis plants. Journal of Biological Chemistry. 2020, 295:8064-8077.
8. Qinghui Han, Tao Li, Lifeng Zhang, Lynnette M.A. Dirk, Bruce Downie, Tianyong Zhao*, 2015, Functional analysis of the 5’ regulatory region of the maize ALKALINE ALPHA-GALACTOSIDASE1 gene. Plant Molecular Biology Reporter. 33:1361-1370.
9. Lei Gu, Zhaoxue Han, Lifeng Zhang, Bruce Downie, Tianyong Zhao*, 2013, Functional analysis of the 5’ regulatory region of the maize GALACTINOL SYNTHASE2 gene, Plant Science 213：38-45.
10. Tianyong Zhao, J. Willis Corum III, Jeffrey Mullen, Robert B. Meeley, Timothy Helentjaris, David Martin, and Bruce Downie, 2006, An alkaline a-galactosidase transcript is present in maize seeds and cultured embryo cells, and accumulates during stress. Seed Science Research. 16:107-121.
11. Tianyong Zhao, Richard Thacker, J. Willis Corum III, John C. Snyder, Robert B. Meeley, Ralph Obendorf, and Bruce Downie, 2004, Expression of the maize GALACTINOL SYNTHASE gene family. I) Expression of two different genes during seed development and germination. Physiol. Plant. 121(4):634-646.
12. Tianyong Zhao, David Martin, Robert B. Meeley, and Bruce Downie, 2004, Expression of the maize GALACTINOL SYNTHASE gene family. II) Kernel abscission, environmental stress and myo-inositol influences accumulation of transcript in developing seeds and callus cells. Physiol. Plant .121 (4): 647-655.
13. Tianyong Zhao, Robert Meeley, and Bruce Downie, 2003, Aberrant processing of a Maize GALACTINOL SYNTHASE transcript is caused by heat stress. Plant Science. 165:245-256.
四、主合手课题
1. 国度当然基金
2. 国度科技公关要害专项要点名堂
3. 陕西省当然基金
关联神气：tzhao2@nwsuaf.edu.cn